Genomics WA

Overview of Services

Genomics WA is a genomics service provision laboratory in Perth, Australia. We provide cutting edge genomics and data analysis services to biomedical and non-biomedical researchers, our services are listed below.  Our laboratories are located on two sites on the QEII campus. Our DNA and RNA library preparation and Illumina sequencing laboratories are located on the 6th floor of the Perkins building and our Single cell Laboratory, long read sequencing laboratory and GWA TKI offices are located on the 6th and 7th floors of the Telethon Kids Institute.

For enquiries and quotations, contact us on Genomics.WA@telethonkids.org.au   OR 

Register on iLab to submit a quotation request

 

Services on offer

Library preparation and sequencing

1. RNAseq (polyA enrichment)

2. WGS (mechanical fragmentation)

3. WGS (enzymatic fragmentation)

4. 3' end GEX from single cells on 10x Chromium

5. 3' end GEX with feature barcoding on 10x Chromium

6. 5' end GEX from single cells on 10x Chromium

7. 5' GEX with V(D)J sequencing from single cells on 10x Chromium

8. 5' GEX with V(D)J sequencing and feature barcoding from single cells on 10x Chromium

9. 5' GEX with feature barcoding from single cells on 10x Chromium

NGS services on the following Illumina sequencers

1. NovaSeq6000

2. NextSeq500

3. MiniSeq

4. MiSeq

5. iSeq

Bioinformatics data analysis services with set end points for libraries prepared and sequenced in our lab

1. For RNAseq: Analysis endpoint- Alignment and post alignment QC, Output is aligned files and post alignment QC 

2. For RNAseq: Analysis endpoint-List of DEGs and pathway analysis, Output is aligned files, post alignment QC, list of DEGs,  hierarchical clustering, PCA plots, basic pathway analysis and a report

3. For single cell workflows: Analysis endpoint: Cell Ranger analysis, Output is cell clusters called with lists of DEGs between clusters viewable on Loupe browser

 


Our laboratories are located at multiple sites on the QEII Campus. 

Sample submission is by appointment only, please see below locations for sample drop off based on service required

Drop off sample  Where Location
Cells for Single cell library preparation on Chromium 10x Single Cell Laboratory Room 633, Level 6 Telethon Kids Institute
Samples for MiniSeq sequencing MiniSeq Laboratory Room 633, Level 6 Telethon Kids Institute
Samples for long read library preparation and sequencing on Sequel II PacBio Sequel II Laboratory Room 756, Level 7 Telethon Kids Institute
Samples for library preparation from DNA, RNA Pre PCR laboratory Level 6 Perkins Building
Samples for Illumina Sequencing (except MiniSeq) Post PCR laboratory Level 6 Perkins Building

 

 

Equipment use Locations

Instrument Location
Tapestation 4200 HPIMR QC area, Level 6 Perkins Building
Qubit TKI Room 633, Level 6, Telethon Kids Institute
Qubit HPIMR QC area, Level 6 Perkins Building
Vacuum dryer Pre PCR, HPIMR  Pre-PCR laboratory, Level 6 Perkins Building
Vacuum dryer Post PCR, HPIMR  Post-PCR laboratory, Level 6 Perkins Building
Covaris (coming soon) Pre-PCR laboratory, Level 6 Perkins Building
Tapestation 4200 TKI Room 633, Level 6 Telethon Kids Institute
Bioanalyser for DNA Room 633, Level 6 Telethon Kids Institute
Bioanalyser for RNA Room 633, Level 6 Telethon Kids Institute

 

Our team

Name Role HPI phone TKI phone Email
Prof. Alka Saxena Director +61 (08) 6151 0763 +61 (08) 6319 1110 alka.saxena@perkins.org.au
Dr Munir Iqbal Scientific Officer (wet lab)   +61 (08) 6319 1445 Munir.Iqbal@telethonkids.org.au
Mr Jake Day Scientific Officer (wet lab)     Jake.Day@telethonkids.oreg.au
TBC Scientific Officer (wet lab)      
Mr Danny Harbeson Bioinformatics support (dry lab)     dannyharbeson@gmail.com


 

Our Funding Partners

Genomics WA gratefully acknowledges funding received in cash and kind from all our funding partners.

Genomics WA is funded by a co-investment from BioPlatforms AustraliaWA Department of Jobs, Tourism, Science and Innovation (JTSI)UWAHarry Perkins Institute of Medical Research and Telethon Kids Institute as part of the NCRIS (National Collaborative Research Infrastructure Strategy) capability.

We are grateful for the support of the Perron Foundation and The Marshall Center for the donation of $1 million, which has enabled the purchase of the PacBio Sequel II, a long-read sequencing platform.

We gratefully acknowledge the support from the Australian Cancer Research Foundation for the Centre for Advanced Cancer Genomics which has made available the Illumina Novaseq6000 and Nanostring sprint systems for the use of Genomics WA.

 

Contacts

Name Role Phone Email Location
Alka Saxena
Director, Genomics WA
 
Phone +61 6151 0763 and +61 6319 1110
 
alka.saxena@perkins.org.au
 

 

Available Equipment and Resources

Name Price